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KMID : 0352720070310040203
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Journal of Ginseng Research
2007 Volume.31 No. 4 p.203 ~ p.209
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Antioxidant activity of white ginseng extracts prepared by enzyme treatment on V79-4 cells induced by oxidative stress
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Kim Young-Chan
Yim Joo-Hyuk
Rho Jeong-Hae
Cho Chang-Won
Rhee Young-Kyoung
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Abstract
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This study examined the extraction yields, total phenolic compounds content and the antioxidant activities on V79-4 cells of white ginseng extracts prepared by enzyme treatment. Yields of crude extract were 29.5-76%, and total phenolic compounds content showed 0.45-2.2% according to enzyme treatments. Pectinase treatment group showed the highest values of extraction yields and total phenolic compounds content. Pectinase and a-amylase treatment groups protected V79-4 cell viability(above 50%) against H₂O₂-induced oxidative damage. In the result of antioxidant enzyme activity evaluation in cells, enzyme treatments did not show the significant difference of SOD activity (p＞0.05). However, pectinase treatment group exhibited increased CAT and GPx activities (p＞0.05). Also, pectinase and protease treatment group inhibited MDA formation (＞50%) in the lipid peroxidation protection experiment
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KEYWORD
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Korean ginseng, enzyme treatment, antioxidant activity, V79-4
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